Coding
fuGFP-link
Part:BBa_K4488013:Design
Designed by: Donovan Wu, Jackie Yau, Oliver Nicholls, Jasmin Li Group: iGEM22_Sydney_Australia (2022-09-30)
Fusion of free-use GFP with CBDcipA (cellulose-binding domain) at the C-terminal end with a linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 163
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A 45bp linker (BBa_K4488006 ) was inserted between fuGFP and CBDcenA. A 6bp cloning scar (“accgcc”) was also inserted downstream of the linker which is found in BBa_K1321341.
Source
The design of the part was based on our previous construct BBa_K4488009 . The fuGFP sequence can be found here (BBa_K3814004).